| Acute Stress |
Measured impact of blood sampling on metabolite levels. For steady state plasma labeling, mice were infused with stable isotope tracers for 2.5 hrs. Mice fasted 6 hrs before sampling. First, an arterial sample was collected via catheter, then a sample from the tail was collected by snip, then a second arterial sample was collected. The results of these experiments were published in "Impact of acute stress on murine metabolomics and metabolic flux, PNAS 2023". |
C57BL/6N |
pyruvate-[13C3][270],
alanine-[13C3,15N1][25],
glycerol-[13C3][120],
succinate-[13C4][2],
citrate-[13C6][5.5],
glucose-[13C6][110];methionine-[13C5,15N1][4.6];proline-[13C5,15N1][5.5];serine-[13C3,15N1][13];valine-[13C5,15N1][11],
3-hydroxybutyrate-[13C4][32];L-cystine-[13C6,15N2][28];asparagine-[13C4,15N2][3.5];malate-[13C4][1.2];taurine-[13C2,15N1][11],
glycerol-[13C3][120];histidine-[13C6,15N3][2];isoleucine-[13C6,15N1][9];lysine-[13C6,15N2][12];phenylalanine-[13C9,15N1][5];threonine-[13C4,15N1][6.8];tryptophan-[13C11,15N2][2.2],
a-ketoglutarate-[13C5][4];acetate-[1,2-13C2][43];arginine-[13C6,15N4][4.6];aspartate-[13C4,15N1][2.8];glycine-[13C2,15N1][16];leucine-[13C6,15N1][11],
creatine-[1-13C1][1.7],
acetate-[1,2-13C2][43],
histidine-[13C6,15N3][2],
glycine-[13C2,15N1][16],
glutamine-[13C5,15N2][45],
6EAAs (MFWKHT) {histidine-[13C6][5];lysine-[13C6][11.6];methionine-[13C5][7];phenylalanine-[13C9][9];threonine-[13C4][7.7];tryptophan-[13C11][2.6]},
serine-[13C3,15N1][13],
lactate-[13C3][160],
tyrosine-[13C9,15N1][2.4],
3-hydroxybutyrate-[13C4][32],
glutamate-[13C5,15N1][4.5];leucine-[13C6,15N1][11],
C16:0-[13C16][2],
C18:2-[13C18][2],
glutamate-[13C5,15N1][4.5] |
alanine,
leucine,
isoleucine,
glucose,
glutamine,
valine,
succinate,
serine,
pyruvate,
proline,
methionine,
malate,
glycine,
citrate,
3-hydroxybutyrate,
C16:0,
C18:2,
a-ketoglutarate,
asparagine,
creatine,
glutamate,
glycerol,
lysine,
phenylalanine,
threonine,
tryptophan,
aspartate,
tyrosine,
lactate,
arginine,
histidine,
taurine,
L-cystine,
acetate |
C, C+N |
no stress, stress |
Won Dong Lee, Lingfan Liang |
23 Infusates
pyruvate-[13C3][270],
alanine-[13C3,15N1][25],
glycerol-[13C3][120],
succinate-[13C4][2],
citrate-[13C6][5.5],
glucose-[13C6][110];methionine-[13C5,15N1][4.6];proline-[13C5,15N1][5.5];serine-[13C3,15N1][13];valine-[13C5,15N1][11],
3-hydroxybutyrate-[13C4][32];L-cystine-[13C6,15N2][28];asparagine-[13C4,15N2][3.5];malate-[13C4][1.2];taurine-[13C2,15N1][11],
glycerol-[13C3][120];histidine-[13C6,15N3][2];isoleucine-[13C6,15N1][9];lysine-[13C6,15N2][12];phenylalanine-[13C9,15N1][5];threonine-[13C4,15N1][6.8];tryptophan-[13C11,15N2][2.2],
a-ketoglutarate-[13C5][4];acetate-[1,2-13C2][43];arginine-[13C6,15N4][4.6];aspartate-[13C4,15N1][2.8];glycine-[13C2,15N1][16];leucine-[13C6,15N1][11],
creatine-[1-13C1][1.7],
acetate-[1,2-13C2][43],
histidine-[13C6,15N3][2],
glycine-[13C2,15N1][16],
glutamine-[13C5,15N2][45],
6EAAs (MFWKHT) {histidine-[13C6][5];lysine-[13C6][11.6];methionine-[13C5][7];phenylalanine-[13C9][9];threonine-[13C4][7.7];tryptophan-[13C11][2.6]},
serine-[13C3,15N1][13],
lactate-[13C3][160],
tyrosine-[13C9,15N1][2.4],
3-hydroxybutyrate-[13C4][32],
glutamate-[13C5,15N1][4.5];leucine-[13C6,15N1][11],
C16:0-[13C16][2],
C18:2-[13C18][2],
glutamate-[13C5,15N1][4.5]
|
91 |
3 |
189 |
586 |