Animal Treatment Protocols

Name Category Description
BYL-719 animal_treatment Mice with a left flank KPC tumor (~300-600mm^3) were fasted for 6h, and then oral gavaged with 45mg/kg BYL-719 (PI3K inhibitor) followed by immediately starting infusion of U-13C-Valine for 2h.
BYL719 via acute injection animal_treatment Mice were injected i.p. with BYL719 (15 mg/kg) dissolved in Cremaphor EL.
cold 4C acute animal_treatment animal was exposed to 4C for 6 total hours (infusion ended at 6 hours of cold exposure)
cold 6C chronic animal_treatment animal was housed at 6C for 4 weeks prior to the day of infusion, and the infusion was carried out in same temperature
control carboxymethylcellulose animal_treatment Mice with a left flank KPC tumor (~300-600mm^3) were fasted for 6h, and then oral gavaged with carboxymethylcellulose (vehicle control) followed by immediately starting infusion of U-13C-Valine for 2h. This is a control for the BYL-719 treatment.
control sucrose water plus saline infusion animal_treatment Animal was on 20% sucrose drinking water for 4 weeks. Animal received 50 mM 13C-glycine infusion for ~48 hours. This is a control for the sucrose water plus high glycine infusion treatment.
control vehicle for T3 via drinking water animal_treatment Drinking water with 20 uM NaOH dissolved in 0.01% BSA. This is a vehicle control for the T3 via drinking water treatment.
control vehicle with Cremaphor EL via acute injection animal_treatment Mice were injected i.p. with vehicle (5% DMSO, 10% Cremaphor EL, 85% 100 mM sodium bicarbonate pH 9.0). This is a control for MG132 via acute injection and BYL719 via acute injection treatments.
control vehicle with saline via acute injection animal_treatment Mice were injected i.p. with saline. This is a control for HCQ via acute injection treatment.
EIPA via acute injection animal_treatment Mice were injected i.p. with EIPA (300 ug/mouse) at t=130 minutes.
HCQ via acute injection animal_treatment Mice were injected i.p. with hydroxychloroquine (50 mg/kg) dissolved in saline.
MG132 via acute injection animal_treatment Mice were injected i.p. with MG132 (12 mg/kg) dissolved in Cremaphor EL.
no stress animal_treatment sample was collected prior to acute induction of stress (by tail clip)
no treatment animal_treatment no manipulation besides what is already described in other fields
perturbative KR infusion animal_treatment Perturbative rate of lysine and arginine infusion caused by incorrect settings in infusion pump
stress animal_treatment sample was collected after stress was induced (by tail clip)
sucrose water plus high glycine infusion animal_treatment Animal was on 20% sucrose drinking water for 4 weeks. Animal received 50 mM 13C-glycine & 450 mM 12C-glycine (500 mM glycine total) infusion for ~48 hours.
T3 via drinking water animal_treatment Drinking water with 0.5 mg/L T3 (Triiodothyronine) dissolved in 0.01% BSA. Provided for a minimum of 2 weeks before experiment.
thermoneutrality chronic animal_treatment animal was housed at thermoneutrality for 4 weeks prior to the day of infusion, and the infusion was carried out in same temperature