Liquid Chromoatography Protocol Details - polar-reversed-phase-ion-pairing-25-min
| Name | polar-reversed-phase-ion-pairing-25-min |
| Method | polar-reversed-phase-ion-pairing |
| Run Legnth (m) | 25 |
| Description | This method involves the analysis of polar metabolites using reversed-phase ion pairing chromatography on C18 columns, coupled with high resolution mass spectrometry. It provides good separation and detection for polar metabolites in negative ion mode, in particular phosphate metabolites. |