Liquid Chromoatography Protocols

Name Method Run Length (m) Description
lipid-reversed-phase-25-min lipid-reversed-phase 25 This method involves the analysis of lipids using reversed-phase chromatography on C18 columns, coupled with high resolution mass spectrometry. It is mainly used for the analysis of lipids.
polar-HILIC-25-min polar-HILIC 25 This method involves the analysis of polar metabolites using HILIC on Waters Amide columns with basic buffer, coupled with high resolution mass spectrometry
polar-reversed-phase-25-min polar-reversed-phase 25 This method involves the analysis of polar metabolites using C18 columns with acidic buffer, coupled with high resolution mass spectrometry. It is mainly used for the analysis of amino acids and derivatives.
polar-reversed-phase-ion-pairing-25-min polar-reversed-phase-ion-pairing 25 This method involves the analysis of polar metabolites using reversed-phase ion pairing chromatography on C18 columns, coupled with high resolution mass spectrometry. It provides good separation and detection for polar metabolites in negative ion mode, in particular phosphate metabolites.
unknown unknown None Liquid chromatography methodology was not provided or is unavailable.